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İLETİŞİM

Turgut Özal Tıp Merkezi Dergisi

2017, Cilt 24, Sayı 3

Evaluation of IL-32 levels in gingival tissue and serum of experimental periodontitis model

Figen Ongoz Dede1, Umut Balli2, Mustafa Cenk Durmuslar3, Seyma Bozkurt Dogan4, Bahattin Avci5, Bulent Ayas5, Ozgur Korhan Tuncel6

1Ordu University, Faculty of Dentistry, Department of Periodontology, Ordu, Turkey

2Bulent Ecevit University, Faculty of Dentistry, Department of Periodontology, Zonguldak, Turkey

3Bulent Ecevit University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Zonguldak, Turkey

4Yildirim Beyazit University, Faculty of Dentistry, Department of Periodontology, Ankara Turkey

5Ondokuzmayıs University, Faculty of Medicine, Department of Biochemistry, Samsun, Turkey

6Ondokuzmayıs University, Faculty of Medicine, Department of Histology and Embryology, Samsun, Turkey

Abstract

Aim: Interleukin (IL)-32, a recently discovered proinflammatory cytokine, is demonstrated in several infectious diseases. The goal of this study is to investigate the levels of IL-6, IL-10 and IL-32 in gingival tissue and serum of rats with experimental periodontitis.

Material and Methods: Experimental periodontitis was induced by placing a silk ligature around the cervix of both sides of mandibular first molars in each male rat except for control group (Group 1). Thirty male Wistar albino rats were randomly divided into three groups of ten animals each as experimental periodontitis groups (Group 2, ligated for seven days; Group 3, ligated for fourteen days) and periodontally healthy control group (Group 1). At the end of experimental period, rats were sacrificed, and histomorphometric analyses were performed on the mandibles. IL-32, IL-10 and IL-6 levels were measured in gingival tissue and serum samples by ELISA.

Results: Alveolar bone and attachment loss were statistically higher in all experimental groups than those in control group (P<0.001). It was found that the levels of IL-32 and IL-6 (P<0.01) and IL-10 (P<0.05) in gingival tissues were higher in Groups 2 and 3 than those in Group 1 except for IL-32 and IL-10 levels in Group 3. There was a positive correlation between levels of IL-32 and IL-6 in the serum and gingival tissues in all groups (P<0.05).

Conclusion: The present results reveal that IL-32 values are locally increased in periodontitis. Proinflammatory cytokines properties, which are linked to periodontal tissue destruction, are also associated with IL-32.

Keywords: Interleukin-32; Experimental Periodontitis; Gingiva; Serum.

Özet Abstract Tam Makale/PDF
 
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